NSJ Bioreagents

HK1 Antibody / Hexokinase 1

Product Code:
 
NSJ-F54454
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Hexokinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human 1) MCF7 and 2) rat brain lysate with Hexokinase antibody. Predicted molecular weight ~102 kDa.
2 / 4
Western blot testing of rat 1) C2C12 and 2) brain lysate with Hexokinase antibody. Predicted molecular weight ~102 kDa.
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Hexokinase antibody.
4 / 4
IHC testing of FFPE human tonsil tissue with Hexokinase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Western blot testing of human 1) MCF7 and 2) rat brain lysate with Hexokinase antibody. Predicted molecular weight ~102 kDa.
Western blot testing of rat 1) C2C12 and 2) brain lysate with Hexokinase antibody. Predicted molecular weight ~102 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Hexokinase antibody.
IHC testing of FFPE human tonsil tissue with Hexokinase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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CodeSizePrice
NSJ-F54454-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54454-0.4ML0.4 ml£502.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Hexokinase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. The hexokinase gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of the hexokinase gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. HK1 encodes the ubiquitously expressed isoform. Its 5' end includes an exon which is unique to this transcript and which encodes a distinct N-terminus that contains the porin binding domain (PBD). The porin binding domain mediates association with the mitochondrial membrane.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 78-108 from the human protein was used as the immunogen for the Hexokinase antibody.
Limitation:
This Hexokinase antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
P19367