NSJ Bioreagents

SCG10 Antibody / STMN2

Product Code:
 
NSJ-F54395
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the STMN2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
IHC testing of FFPE human brain tissue with STMN2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
IHC testing of FFPE human liver tissue with STMN2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
Immunofluorescent staining of fixed and permeabilized human SH-SY5Y cells with STMN2 antibody (green) and anti-Actin (red).
4 / 5
Western blot testing of 1) human brain, 2) mouse brain and 3) SH-SY5Y lysate with STMN2 antibody. Predicted molecular weight ~21 kDa.
5 / 5
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with STMN2 antibody; Blue=isotype control, Green= STMN2 antibody.
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IHC testing of FFPE human brain tissue with STMN2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human liver tissue with STMN2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human SH-SY5Y cells with STMN2 antibody (green) and anti-Actin (red).
Western blot testing of 1) human brain, 2) mouse brain and 3) SH-SY5Y lysate with STMN2 antibody. Predicted molecular weight ~21 kDa.
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with STMN2 antibody; Blue=isotype control, Green= STMN2 antibody.

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CodeSizePrice
NSJ-F54395-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54395-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the STMN2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Regulator of microtubule stability. When phosphorylated by MAPK8, stabilizes microtubules and consequently controls neurite length in cortical neurons. In the developing brain, negatively regulates the rate of exit from multipolar stage and retards radial migration from the ventricular zone (By similarity).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 82-116 from the human protein was used as the immunogen for the STMN2 antibody.
Limitation:
This STMN2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q93045