NSJ Bioreagents

UBA1 Antibody / E1 Ubiquitin activating enzyme

Product Code:
 
NSJ-F54754
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Ubiquitin-like modifier-activating enzyme 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
IHC testing of FFPE human hepatocellular carcinoma tissue with Ubiquitin-like modifier-activating enzyme 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 3
Western blot testing of human HL60 cell lysate with Ubiquitin-like modifier-activating enzyme 1 antibody. Expected molecular weight: ~118/114 kDa (isoforms a/b).
3 / 3
Western blot testing of mouse stomach tissue lysate with Ubiquitin-like modifier-activating enzyme 1 antibody. Expected molecular weight: ~118/114 kDa (isoforms a/b).
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IHC testing of FFPE human hepatocellular carcinoma tissue with Ubiquitin-like modifier-activating enzyme 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HL60 cell lysate with Ubiquitin-like modifier-activating enzyme 1 antibody. Expected molecular weight: ~118/114 kDa (isoforms a/b).
Western blot testing of mouse stomach tissue lysate with Ubiquitin-like modifier-activating enzyme 1 antibody. Expected molecular weight: ~118/114 kDa (isoforms a/b).

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CodeSizePrice
NSJ-F54754-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54754-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Ubiquitin-like modifier-activating enzyme 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
UBE1 / UBA1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1026-1058 from the human protein was used as the immunogen for the Ubiquitin-like modifier-activating enzyme 1 antibody.
Limitation:
This Ubiquitin-like modifier-activating enzyme 1 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Purified
Species Reactivity :
Human, Mouse
Uniprot #:
P22314