NSJ Bioreagents

PDK2 Antibody / Pyruvate dehydrogenase kinase 2

Product Code:
 
NSJ-F54683
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Pyruvate dehydrogenase kinase 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC testing of FFPE mouse heart tissue with Pyruvate dehydrogenase kinase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human HepG2 cell lysate with Pyruvate dehydrogenase kinase 2 antibody. Predicted molecular weight ~46 kDa.
3 / 4
Western blot testing of mouse heart cell lysate with Pyruvate dehydrogenase kinase 2 antibody. Predicted molecular weight ~46 kDa.
4 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Pyruvate dehydrogenase kinase 2 antibody. Predicted molecular weight ~46 kDa.
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IHC testing of FFPE mouse heart tissue with Pyruvate dehydrogenase kinase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HepG2 cell lysate with Pyruvate dehydrogenase kinase 2 antibody. Predicted molecular weight ~46 kDa.
Western blot testing of mouse heart cell lysate with Pyruvate dehydrogenase kinase 2 antibody. Predicted molecular weight ~46 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Pyruvate dehydrogenase kinase 2 antibody. Predicted molecular weight ~46 kDa.

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CodeSizePrice
NSJ-F54683-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54683-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Pyruvate dehydrogenase kinase 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Pdk2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 78-107 from the mouse protein was used as the immunogen for the Pyruvate dehydrogenase kinase 2 antibody.
Limitation:
This Pyruvate dehydrogenase kinase 2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9JK42