NSJ Bioreagents

Dystroglycan Antibody / DAG

Product Code:
 
NSJ-F54375
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the DAG1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of 1) human HeLa and 2) mouse NIH 3T3 cell lysate with DAG1 antibody. Predicted molecular weight ~97 kDa.
2 / 4
Western blot testing of 1) human SH-SY5Y and 2) mouse NIH 3T3 cell lysate with DAG1 antibody. Predicted molecular weight ~97 kDa.
3 / 4
Western blot testing of human 1) HeLa, 2) MCF7 and 3) SH-SY5Y cell lysate with DAG1 antibody. Predicted molecular weight ~97 kDa.
4 / 4
IHC testing of FFPE human skeletal muscle tissue with DAG1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of 1) human HeLa and 2) mouse NIH 3T3 cell lysate with DAG1 antibody. Predicted molecular weight ~97 kDa.
Western blot testing of 1) human SH-SY5Y and 2) mouse NIH 3T3 cell lysate with DAG1 antibody. Predicted molecular weight ~97 kDa.
Western blot testing of human 1) HeLa, 2) MCF7 and 3) SH-SY5Y cell lysate with DAG1 antibody. Predicted molecular weight ~97 kDa.
IHC testing of FFPE human skeletal muscle tissue with DAG1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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CodeSizePrice
NSJ-F54375-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54375-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the DAG1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 718-747 from the human protein was used as the immunogen for the DAG1 antibody.
Limitation:
This DAG1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q14118