NSJ Bioreagents

COX7A2L Antibody

Product Code:
 
NSJ-F54648
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the COX7A2L antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human HepG2 cell lysate with COX7A2L antibody. Predicted molecular weight ~13 kDa.
2 / 4
Western blot testing of human A549 cell lysate with COX7A2L antibody. Predicted molecular weight ~13 kDa.
3 / 4
Western blot testing of mouse NIH 3T3 cell lysate with COX7A2L antibody. Predicted molecular weight ~13 kDa.
4 / 4
IHC testing of FFPE human breast carcinoma tissue with COX7A2L antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human HepG2 cell lysate with COX7A2L antibody. Predicted molecular weight ~13 kDa.
Western blot testing of human A549 cell lysate with COX7A2L antibody. Predicted molecular weight ~13 kDa.
Western blot testing of mouse NIH 3T3 cell lysate with COX7A2L antibody. Predicted molecular weight ~13 kDa.
IHC testing of FFPE human breast carcinoma tissue with COX7A2L antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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CodeSizePrice
NSJ-F54648-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54648-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the COX7A2L antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein similar to polypeptides 1 and 2 of subunit VIIa in the C-terminal region, and also highly similar to the mouse Sig81 protein sequence. This gene is expressed in all tissues, and upregulated in a breast cancer cell line after estrogen treatment. It is possible that this gene represents a regulatory subunit of COX and mediates the higher level of energy production in target cells by estrogen.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 37-65 from the human protein was used as the immunogen for the COX7A2L antibody.
Limitation:
This COX7A2L antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
O14548