NSJ Bioreagents

BRD9 Antibody

Product Code:
 
NSJ-F54727
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the BRD9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC testing of FFPE human testis tissue with BRD9 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human placenta tissue with BRD9 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of human 1) HeLa, 2) SH-SY5Y and 3) placenta lysate with BRD9 antibody. Predicted molecular weight ~67 kDa.
4 / 4
Western blot testing of mouse Neuro-2a cell lysate with BRD9 antibody. Predicted molecular weight ~67 kDa.
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IHC testing of FFPE human testis tissue with BRD9 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human placenta tissue with BRD9 antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Western blot testing of human 1) HeLa, 2) SH-SY5Y and 3) placenta lysate with BRD9 antibody. Predicted molecular weight ~67 kDa.
Western blot testing of mouse Neuro-2a cell lysate with BRD9 antibody. Predicted molecular weight ~67 kDa.

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CodeSizePrice
NSJ-F54727-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54727-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the BRD9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Bromodomain-containing protein 9 plays a role in chromatin remodeling and regulation of transcription. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 3-29 from the human protein was used as the immunogen for the BRD9 antibody.
Limitation:
This BRD9 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q9H8M2