NSJ Bioreagents

USP17L24 Antibody

Product Code:
 
NSJ-F54295
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the USP17L24 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
Western blot testing of human 1) U-87 MG, 2) MDA-MB-231 and 3) U-251 MG cell lysate with USP17L24 antibody. Predicted molecular weight ~60 kDa.
2 / 5
Western blot testing of human HepG2 cell lysate with USP17L24 antibody. Predicted molecular weight ~60 kDa.
3 / 5
Immunofluorescent staining of fixed and permeabilized human U-251 MG cells with USP17L24 antibody (green) and anti-Actin (red).
4 / 5
IHC testing of FFPE human brain tissue with USP17L24 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of fixed and permeabilized human HepG2 cells with USP17L24 antibody; Blue=isotype control, Green= USP17L24 antibody.
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Western blot testing of human 1) U-87 MG, 2) MDA-MB-231 and 3) U-251 MG cell lysate with USP17L24 antibody. Predicted molecular weight ~60 kDa.
Western blot testing of human HepG2 cell lysate with USP17L24 antibody. Predicted molecular weight ~60 kDa.
Immunofluorescent staining of fixed and permeabilized human U-251 MG cells with USP17L24 antibody (green) and anti-Actin (red).
IHC testing of FFPE human brain tissue with USP17L24 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of fixed and permeabilized human HepG2 cells with USP17L24 antibody; Blue=isotype control, Green= USP17L24 antibody.

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CodeSizePrice
NSJ-F54295-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54295-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25,Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the USP17L24 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
USP17 recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins (By similarity).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 496-524 from the human protein were used as the immunogen for the USP17L24 antibody.
Limitation:
This USP17L24 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q0WX57