NSJ Bioreagents

IKAROS Antibody / IKZF1

Product Code:
 
NSJ-F54538
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the IKAROS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of lysate from multiple myeloma cells treated with DMSO (vehicle) or lenalidomide (degrades IKAROS). Predicted molecular weight ~58 kDa.
2 / 4
Western blot testing of human CEM cell lysate with IKAROS antibody. Predicted molecular weight ~58 kDa.
3 / 4
IHC testing of FFPE human tonsil tissue with IKAROS antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human CEM cells with IKAROS antibody; Blue=isotype control, Green= IKAROS antibody.
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Western blot testing of lysate from multiple myeloma cells treated with DMSO (vehicle) or lenalidomide (degrades IKAROS). Predicted molecular weight ~58 kDa.
Western blot testing of human CEM cell lysate with IKAROS antibody. Predicted molecular weight ~58 kDa.
IHC testing of FFPE human tonsil tissue with IKAROS antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human CEM cells with IKAROS antibody; Blue=isotype control, Green= IKAROS antibody.

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CodeSizePrice
NSJ-F54538-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54538-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:2000, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the IKAROS antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 415-444 from the human protein was used as the immunogen for the IKAROS antibody.
Limitation:
This IKAROS antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q13422