NSJ Bioreagents

NANOS1 Antibody

Product Code:
 
NSJ-F54684
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the NANOS1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human T-47D cell lysate with NANOS1 antibody. Predicted molecular weight: ~30 kDa but routinely observed at 35~40 kDa.
2 / 4
Immunofluorescent staining of human HeLa cells with NANOS1 antibody (red) and DAPI nuclear stain (blue).
3 / 4
IHC testing of FFPE human breast cancer tissue with NANOS1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HeLa cells with NANOS1 antibody; Blue=isotype control, Green= NANOS1 antibody.
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Western blot testing of human T-47D cell lysate with NANOS1 antibody. Predicted molecular weight: ~30 kDa but routinely observed at 35~40 kDa.
Immunofluorescent staining of human HeLa cells with NANOS1 antibody (red) and DAPI nuclear stain (blue).
IHC testing of FFPE human breast cancer tissue with NANOS1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HeLa cells with NANOS1 antibody; Blue=isotype control, Green= NANOS1 antibody.

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CodeSizePrice
NSJ-F54684-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54684-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the NANOS1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NANOS1 may regulate translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 263-292 from the human protein was used as the immunogen for the NANOS1 antibody.
Limitation:
This NANOS1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q8WY41