NSJ Bioreagents

ZIC3 Antibody

Product Code:
 
NSJ-F54773
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the ZIC3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human HL60 cell lysate with ZIC3 antibody. Predicted molecular weight ~51 kDa.
2 / 4
Western blot testing of human CCRF-CEM cell lysate with ZIC3 antibody. Predicted molecular weight ~51 kDa.
3 / 4
IHC testing of FFPE human brain tissue with ZIC3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human CCRF-CEM cells with ZIC3 antibody; Blue=isotype control, Green= ZIC3 antibody.
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Western blot testing of human HL60 cell lysate with ZIC3 antibody. Predicted molecular weight ~51 kDa.
Western blot testing of human CCRF-CEM cell lysate with ZIC3 antibody. Predicted molecular weight ~51 kDa.
IHC testing of FFPE human brain tissue with ZIC3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human CCRF-CEM cells with ZIC3 antibody; Blue=isotype control, Green= ZIC3 antibody.

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CodeSizePrice
NSJ-F54773-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54773-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 1:50-1:100 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the ZIC3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 239-267 from the human protein was used as the immunogen for the ZIC3 antibody.
Limitation:
This ZIC3 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
O60481