NSJ Bioreagents

SMAC/Diablo Antibody

Product Code:
 
NSJ-F54888
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the SMAC/Diablo antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC testing of FFPE human testis tissue with SMAC/Diablo antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human 1) HeLa and 2) Jurkat cell lysate with SMAC/Diablo antibody. Predicted molecular weight: 21-27 kDa.
3 / 4
Western blot testing of human T-47D cell lysate with SMAC/Diablo antibody. Predicted molecular weight: 21-27 kDa.
4 / 4
Flow cytometry testing of human MDA-MB-231 cells with SMAC/Diablo antibody; Blue=isotype control, Green= SMAC/Diablo antibody.
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IHC testing of FFPE human testis tissue with SMAC/Diablo antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) HeLa and 2) Jurkat cell lysate with SMAC/Diablo antibody. Predicted molecular weight: 21-27 kDa.
Western blot testing of human T-47D cell lysate with SMAC/Diablo antibody. Predicted molecular weight: 21-27 kDa.
Flow cytometry testing of human MDA-MB-231 cells with SMAC/Diablo antibody; Blue=isotype control, Green= SMAC/Diablo antibody.

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CodeSizePrice
NSJ-F54888-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54888-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the SMAC/Diablo antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
SMAC is an inhibitor of apoptosis protein (IAP)-binding protein. This mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 211-239 from the human protein was used as the immunogen for the SMAC/Diablo antibody.
Limitation:
This SMAC/Diablo antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q9NR28