NSJ Bioreagents

Peptidase inhibitor 15 Antibody / PI15

Product Code:
 
NSJ-F54668
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the PI15 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human 1) HeLa and 2) PC-3 cell lysate with PI15 antibody. Predicted molecular weight ~29 kDa.
2 / 4
Western blot testing of human K562 cell lysate with PI15 antibody. Predicted molecular weight ~29 kDa.
3 / 4
IHC testing of FFPE human breast carcinoma tissue with PI15 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human K562 cells with PI15 antibody; Left=isotype control, Right= PI15 antibody.
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Western blot testing of human 1) HeLa and 2) PC-3 cell lysate with PI15 antibody. Predicted molecular weight ~29 kDa.
Western blot testing of human K562 cell lysate with PI15 antibody. Predicted molecular weight ~29 kDa.
IHC testing of FFPE human breast carcinoma tissue with PI15 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human K562 cells with PI15 antibody; Left=isotype control, Right= PI15 antibody.

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CodeSizePrice
NSJ-F54668-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54668-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the PI15 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines, and thus may play a role in the central nervous system. [RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 34-63 from the human protein was used as the immunogen for the PI15 antibody.
Limitation:
This PI15 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O43692