NSJ Bioreagents

Phospho-ERK1/2 Antibody [p-ERK1/2] (T202/Y204)

Product Code:
 
NSJ-F48590
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot the phospho-ERK1/2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 2
Western blot testing of human HeLa and mouse NIH3T3 cells treated with TPA (200nM) and PDGF (100ng/ml) using phospho-ERK1/2 antibody.~
2 / 2
Dot blot analysis of phospho-ERK1/2 antibody. 50ng of Bi-phos-peptide or nonphosrylated peptide per
❮❯

Western blot testing of human HeLa and mouse NIH3T3 cells treated with TPA (200nM) and PDGF (100ng/ml) using phospho-ERK1/2 antibody.~
Dot blot analysis of phospho-ERK1/2 antibody. 50ng of Bi-phos-peptide or nonphosrylated peptide per

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CodeSizePrice
NSJ-F48590-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48590-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Dot blot: 1:500,Western blot: 1:1000
Application Note:
Titration of the phospho-ERK1/2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
MAPK3/ERK1 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This phospho-ERK1/2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT202/pY204 of human ERK1/2.
Limitation:
This phospho-ERK1/2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Species Reactivity (Predicted):
Rat, Drosophila
Uniprot #:
P27361