NSJ Bioreagents

Phospho-NANOG Antibody (pS285)

Product Code:
 
NSJ-F48602
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot the phospho-NANOG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Dot blot analysis of phospho-NANOG antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.
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Dot blot analysis of phospho-NANOG antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.

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CodeSizePrice
NSJ-F48602-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48602-0.4ML0.4 ml£502.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Dot blot: 1:500
Application Note:
Titration of the phospho-NANOG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This phospho-NANOG antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS285 of human Nanog.
Limitation:
This phospho-NANOG antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q9H9S0