NSJ Bioreagents

NRAS Antibody

Product Code:
 
NSJ-F50734
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the NRAS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
IHC analysis of FFPE human lung carcinoma tissue stained with NRAS antibody
2 / 3
NRAS antibody western blot analysis in MCF-7 lysate. Predicted molecular weight ~21 kDa.
3 / 3
NRAS antibody flow cytometric analysis of NCI-H460 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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IHC analysis of FFPE human lung carcinoma tissue stained with NRAS antibody
NRAS antibody western blot analysis in MCF-7 lysate. Predicted molecular weight ~21 kDa.
NRAS antibody flow cytometric analysis of NCI-H460 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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CodeSizePrice
NSJ-F50734-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50734-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the NRAS antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 147-179 from the human protein was used as the immunogen for this NRAS antibody.
Limitation:
This NRAS antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Pig
Uniprot #:
P01111