NSJ Bioreagents

MGMT Antibody

Product Code:
 
NSJ-F50325
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the MGMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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MGMT antibody western blot analysis in Jurkat lysate.
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IHC analysis of FFPE human placenta with MGMT antibody
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Flow cytometric analysis of CEM cells using MGMT antibody (bottom histogram) compared to a <a href=../tds/rabbit-igg-isotype-control-polyclonal-antibody-n1001>negative control</a> (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

MGMT antibody western blot analysis in Jurkat lysate.
IHC analysis of FFPE human placenta with MGMT antibody
Flow cytometric analysis of CEM cells using MGMT antibody (bottom histogram) compared to a <a href=../tds/rabbit-igg-isotype-control-polyclonal-antibody-n1001>negative control</a> (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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CodeSizePrice
NSJ-F50325-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50325-0.4ML0.4 ml£502.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the MGMT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. This protein repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the protein is irreversibly inactivated.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 156-182 from the human protein was used as the immunogen for this MGMT antibody.
Limitation:
This MGMT antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P16455