NSJ Bioreagents

C5 Antibody

Product Code:
 
NSJ-F51211
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the C5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Western blot analysis of C5 antibody and HL-60 lysate
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IHC analysis of FFPE human breast carcinoma with C5 antibody
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Flow cytometric analysis of MDA-231 cells using C5 antibody (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Western blot analysis of C5 antibody and HL-60 lysate
IHC analysis of FFPE human breast carcinoma with C5 antibody
Flow cytometric analysis of MDA-231 cells using C5 antibody (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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CodeSizePrice
NSJ-F51211-0.08ML0.08 ml£260.00
Quantity:
NSJ-F51211-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the C5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
C5 is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 253-281 from the human protein was used as the immunogen for this C5 antibody.
Limitation:
This C5 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P01031