NSJ Bioreagents

BRAF Antibody

Product Code:
 
NSJ-F50795
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the BRAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Western blot analysis of lysate from MCF-7 cell line using BRAF antibody. Predicted size 85-95 kDa~
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IHC analysis of FFPE human brain tissue stained with BRAF antibody
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Western blot analysis of lysate from MCF-7 cell line using BRAF antibody. Predicted size 85-95 kDa~
IHC analysis of FFPE human brain tissue stained with BRAF antibody

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CodeSizePrice
NSJ-F50795-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50795-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the BRAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 580-609 from the human protein was used as the immunogen for this BRAF antibody.
Limitation:
This BRAF antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Chicken, Mouse
Uniprot #:
P15056