NSJ Bioreagents

CAD Protein Antibody

Product Code:
 
NSJ-F41205
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the CAD antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
CAD antibody western blot analysis in Jurkat lysate. Predicted molecular weight ~243 kDa.
2 / 3
CAD antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma.
3 / 3
CAD antibody flow cytometric analysis of Jurkat cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated donkey-anti-rabbit secondary Ab was used for the analysis.
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CAD antibody western blot analysis in Jurkat lysate. Predicted molecular weight ~243 kDa.
CAD antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma.
CAD antibody flow cytometric analysis of Jurkat cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated donkey-anti-rabbit secondary Ab was used for the analysis.

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CodeSizePrice
NSJ-F41205-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41205-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the CAD antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 780-809 from the human protein was used as the immunogen for this CAD antibody.
Limitation:
This CAD antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse
Uniprot #:
P27708