NSJ Bioreagents

Annexin V Antibody

Product Code:
 
NSJ-F49706
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the Annexin V antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Western blot analysis of Annexin V antibody and (1) HepG2, (2) A2058, (3) T47D lysate. Predicted molecular weight ~36 kDa.
2 / 3
Annexin V antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma.
3 / 3
Annexin V antibody flow cytometric analysis of A2058 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Western blot analysis of Annexin V antibody and (1) HepG2, (2) A2058, (3) T47D lysate. Predicted molecular weight ~36 kDa.
Annexin V antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma.
Annexin V antibody flow cytometric analysis of A2058 cells (green) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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CodeSizePrice
NSJ-F49706-0.08ML0.08 ml£260.00
Quantity:
NSJ-F49706-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the Annexin V antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Annexin 5 belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1-30 from the human protein was used as the immunogen for this Annexin V antibody.
Limitation:
This Annexin V antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Bovine, Primate
Uniprot #:
P08758