NSJ Bioreagents

NANOG Antibody

Product Code:
 
NSJ-F44247
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the NANOG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Western blot analysis of NANOG antibody and K562 lysate; Predicted molecular weight: 35-45 kDa.
2 / 6
Western blot analysis of NANOG antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2). Predicted molecular weight: 35-45 kDa.
3 / 6
Fluorescent confocal image of SY5Y cells stained with NANOG antibody at 1:50. Immunoreactivity is localized mainly to the nuclei of the cells.
4 / 6
Fluorescent confocal image of HeLa cell stained with NANOG antibody at 1:25. Immunoreactivity is localized to the nucleus.
5 / 6
IHC analysis of FFPE human spleen tissue stained with NANOG antibody
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NANOG antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Western blot analysis of NANOG antibody and K562 lysate; Predicted molecular weight: 35-45 kDa.
Western blot analysis of NANOG antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the human gene (2). Predicted molecular weight: 35-45 kDa.
Fluorescent confocal image of SY5Y cells stained with NANOG antibody at 1:50. Immunoreactivity is localized mainly to the nuclei of the cells.
Fluorescent confocal image of HeLa cell stained with NANOG antibody at 1:25. Immunoreactivity is localized to the nucleus.
IHC analysis of FFPE human spleen tissue stained with NANOG antibody
NANOG antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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CodeSizePrice
NSJ-F44247-0.08ML0.08 ml£260.00
Quantity:
NSJ-F44247-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the NANOG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 15-49 from the human protein was used as the immunogen for this NANOG antibody.
Limitation:
This NANOG antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Primate
Uniprot #:
Q9H9S0