NSJ Bioreagents

Anit-GAPDH Antibody

Product Code:
 
NSJ-F50849
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the anti-GAPDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 6
Western blot analysis of lysate from A375 cell line using anti-GAPDH antibody diluted at 1:500.
2 / 6
Western blot analysis of lysate from HeLa, HUVEC cell line (left to right) using anti-GAPDH antibody diluted at 1:1000 for each lane.
3 / 6
IHC analysis of FFPE human hepatocarcinoma tissue stained with anti-GAPDH antibody
4 / 6
Western blot analysis of anti-GAPDH antibody and 1) A2058, 2) A375, and 3) CEM lysate
5 / 6
Anti-GAPDH antibody confocal immunofluorescent analysis with HeLa cell. Primary antibody (1:20) was followed by FITC-conjugated goat anti-rabbit lgG. FITC emits green fluorescence. DAPI was used as a nuclear counterstain (blue).
6 / 6
Confocal immunofluorescent analysis of anti-GAPDH antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
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Western blot analysis of lysate from A375 cell line using anti-GAPDH antibody diluted at 1:500.
Western blot analysis of lysate from HeLa, HUVEC cell line (left to right) using anti-GAPDH antibody diluted at 1:1000 for each lane.
IHC analysis of FFPE human hepatocarcinoma tissue stained with anti-GAPDH antibody
Western blot analysis of anti-GAPDH antibody and 1) A2058, 2) A375, and 3) CEM lysate
Anti-GAPDH antibody confocal immunofluorescent analysis with HeLa cell. Primary antibody (1:20) was followed by FITC-conjugated goat anti-rabbit lgG. FITC emits green fluorescence. DAPI was used as a nuclear counterstain (blue).
Confocal immunofluorescent analysis of anti-GAPDH antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).

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CodeSizePrice
NSJ-F50849-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50849-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the anti-GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 62-91 from the human protein was used as the immunogen for this anti-GAPDH antibody.
Limitation:
This anti-GAPDH antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P04406