NSJ Bioreagents

EIF4E Antibody

Product Code:
 
NSJ-F47411
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the EIF4E antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
EIF4E antibody flow cytometric analysis of HL-60 cells (bottom histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
2 / 3
IHC analysis of FFPE human testis tissue stained with EIF4E antibody
3 / 3
Western blot analysis of EIF4E antibody and HL-60 lysate. Predicted molecular weight ~27 kDa.
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EIF4E antibody flow cytometric analysis of HL-60 cells (bottom histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
IHC analysis of FFPE human testis tissue stained with EIF4E antibody
Western blot analysis of EIF4E antibody and HL-60 lysate. Predicted molecular weight ~27 kDa.

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CodeSizePrice
NSJ-F47411-0.08ML0.08 ml£260.00
Quantity:
NSJ-F47411-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 27-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This EIF4E antibody was produced from rabbits immunized with a recombinant protein from human VGFR2.
Limitation:
This EIF4E antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P06730