NSJ Bioreagents

B-RAF Antibody

Product Code:
 
NSJ-F50792
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store at 4oC for up to one month. For long term, aliquot the B-RAF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Fluorescent image of C2C12 cell stained with B-RAF antibody at 1:25. Immunoreactivity is localized to the cytoplasm.
2 / 4
Western blot analysis of B-RAF antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (2). Predicted size 85-95 kDa
3 / 4
IHC analysis of FFPE human prostate carcinoma stained with B-RAF antibody
4 / 4
B-RAF antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Fluorescent image of C2C12 cell stained with B-RAF antibody at 1:25. Immunoreactivity is localized to the cytoplasm.
Western blot analysis of B-RAF antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (2). Predicted size 85-95 kDa
IHC analysis of FFPE human prostate carcinoma stained with B-RAF antibody
B-RAF antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

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CodeSizePrice
NSJ-F50792-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50792-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunofluorescence: 1:10-1:50,Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the B-RAF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
BRAF is a human gene, with the protein more formally known as serine/threonine-protein kinase B-Raf. The protein is involved in sending signals inside cells, which are involved in directing cell growth. Drugs that treat cancers driven by BRAF mutations have been developed. Two of these drugs, Vemurafenib and Dabrafenib are approved by FDA for treatment of late-stage melanoma. [Wiki]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 424-453 from the human protein was used as the immunogen for this B-RAF antibody.
Limitation:
This B-RAF antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Species Reactivity (Predicted):
Mouse, Chicken
Uniprot #:
P15056