NSJ Bioreagents

CD8A Antibody

Product Code:
 
NSJ-V2386
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM548
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
CD8a antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
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IHC testing of CD8a antibody (clone SPM548) and FFPE human tonsil tissue.
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IHC testing of CD8a antibody (clone SPM548) and FFPE human tonsil tissue.

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CodeSizePrice
NSJ-V2386-20UG20 ug£264.00
Quantity:
NSJ-V2386-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Variations in protocols, secondaries and substrates may require the CD8a antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
CD8 molecule consists of two chains, termed alpha and beta chain, which are expressed as a disulphide-linked heterodimer or homodimer. CD8 is expressed on T cell subset (cytotoxic/suppressor T cells), thymocytes and NK cells.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Human CD8 recombinant protein was used as immunogen for this CD8a antibody.
Limitation:
This CD8a antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P01732