NSJ Bioreagents

Cytokeratin 18 Antibody

Product Code:
 
NSJ-V9041
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM510
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the anti-Cytokeratin 18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Flow cytometry testing of PFA-fixed human HeLa cells with anti-Cytokeratin 18 antibody (clone SPM510); Red=isotype control, Blue= anti-Cytokeratin 18 antibody.~
2 / 2
IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with a
❮❯

Flow cytometry testing of PFA-fixed human HeLa cells with anti-Cytokeratin 18 antibody (clone SPM510); Red=isotype control, Blue= anti-Cytokeratin 18 antibody.~
IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with a

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CodeSizePrice
NSJ-V9041-20UG20 ug£264.00
Quantity:
NSJ-V9041-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Human breast cancer PMC 42 cells were used as the immunogen for this anti-Cytokeratin 18 antibody.
Limitation:
This anti-Cytokeratin 18 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P05783