S100A8 Antibody

Western blot: 2-4ug/ml

Provided assay concentrations are suggestions only, S100A8 antibody titration may be required for optimal results.

1X PBS, pH 7.4

The S100 family of proteins are found in vertebrates and are characterized by two calcium-binding sites that have a helix-loop-helix conformation. The family of 24 is divided into three subgroups based on function: those with intracellular, intracellular plus extracellular, and extracellular regulatory effects. The calcium-binding protein S100A8, also called MRP8 or Myeloid-related protein 8, and Calgranulin A, forms a heterodimer with S100A9, called Calprotectin. The heterodimer is expressed by granulocytes, monocytes, and early differentiation states of macrophages, and is known to be an important pro-inflammatory mediator in acute and chronic inflammation. S100A8 and 9 are DAMPs, or Damage-associated molecular pattern molecules, and are able to activate receptors of the innate immune system, one being TLR4.

Purified

0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

6279

A recombinant protein fragment from the N-terminal region of human S100A8 was used as the immunogen for this antibody.

This S100A8 antibody is available for research use only.

Protein G affinity chromatography

Human