NSJ Bioreagents

SUMO2/3 Antibody

Product Code:
 
NSJ-V7091
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
S23MT-1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the SUMO2/3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Western blot testing of human HeLa lysate with SUMO2/3 antibody (clone S23MT-1).~
2 / 2
IHC analysis of human tonsil with SUMO2/3 antibody (clone S23MT-1). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
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Western blot testing of human HeLa lysate with SUMO2/3 antibody (clone S23MT-1).~
IHC analysis of human tonsil with SUMO2/3 antibody (clone S23MT-1). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

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CodeSizePrice
NSJ-V7091-20UG20 ug£264.00
Quantity:
NSJ-V7091-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Titering of the SUMO2/3 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
The small ubiquitin-related modifier (SUMO) proteins, which include SUMO1, 2 and 3, belong to the ubiquitin-like protein family. SUMO proteins are synthesized as precursors that undergo processing before conjugation to target proteins. Sumoylation targets for a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. SUMO2/3 contribute to a greater percentage of protein modification than does SUMO1 and they can form polymeric chains. In addition, SUMO3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimer�s disease.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
6613, 6612
Immunogen:
Recombinant human SUMO2 protein was used as the immunogen for this SUMO2/3 antibody. This antibody reacts with both SUMO2 and SUMO3.
Limitation:
This SUMO2/3 antibody is available for research use only.
Localization:
Predominantly nuclear with some cytoplasmic staining
Purity:
Protein G purified
Species Reactivity :
Human