NSJ Bioreagents

GFAP Antibody / Glial Fibrillary Acidic Protein

Product Code:
 
NSJ-V3589
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
rASTRO/789
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant Glial Fibrillary Acidic Protein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Western blot testing of human brain lysate with recombinant Glial Fibrillary Acidic Protein antibody (clone rASTRO/789). Predicted molecular weight ~50 kDa.~
2 / 2
IHC testing of FFPE human cerebellum tissue with recombinant Glial Fibrillary A
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Western blot testing of human brain lysate with recombinant Glial Fibrillary Acidic Protein antibody (clone rASTRO/789). Predicted molecular weight ~50 kDa.~
IHC testing of FFPE human cerebellum tissue with recombinant Glial Fibrillary A

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CodeSizePrice
NSJ-V3589-20UG20 ug£264.00
Quantity:
NSJ-V3589-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Application Note:
The optimal dilution of the recombinant Glial Fibrillary Acidic Protein antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
GFAP isolated from pig spinal cord was used as the immunogen for this recombinant Glial Fibrillary Acidic Protein antibody.
Limitation:
This recombinant Glial Fibrillary Acidic Protein antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Rat
Uniprot #:
P14136