NSJ Bioreagents

AKT1 Antibody

Product Code:
 
NSJ-V3798
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
RASTPK-1
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC testing of FFPE human pancreas with AKT1 antibody (clone RASTPK-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human pancreas with AKT1 antibody (clone RASTPK-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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CodeSizePrice
NSJ-V3798-20UG20 ug£264.00
Quantity:
NSJ-V3798-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
IHC (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The stated application concentrations are suggested starting points. Titration of the AKT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A portion of amino acids 85-189 from the human protein was used as the immunogen for the AKT1 antibody.
Limitation:
This AKT1 antibody is available for research use only.
Localization:
Nucleus, plasma membrane, cytoplasm
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P31749