NSJ Bioreagents

Neurofilament Antibody (-Light) / NF-L / NEFL

Product Code:
 
NSJ-V7122
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MSI27-1
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the Neurofilament antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 1
IHC testing of FFPE human cerebellum with Neurofilament antibody (clone MSI27-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human cerebellum with Neurofilament antibody (clone MSI27-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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CodeSizePrice
NSJ-V7122-20UG20 ug£264.00
Quantity:
NSJ-V7122-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Optimal dilution of the Neurofilament antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. [RefSeq]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant human neurofilament light chain protein was used as the immunogen for the Neurofilament antibody.
Limitation:
This Neurofilament antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Rat
Uniprot #:
P07196