NSJ Bioreagents

CD27 Antibody

Product Code:
 
NSJ-V3680
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
LPFS2/2034R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the recombinant CD27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC testing of FFPE human lymph node with recombinant CD27 antibody (clone LPFS2/2034R). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min.~
2 / 2
SDS-PAGE analysis of purified, BSA-free recombinant CD2
❮❯

IHC testing of FFPE human lymph node with recombinant CD27 antibody (clone LPFS2/2034R). Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min.~
SDS-PAGE analysis of purified, BSA-free recombinant CD2

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CodeSizePrice
NSJ-V3680-20UG20 ug£264.00
Quantity:
NSJ-V3680-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant CD27 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFkB and SAPK/JNK and induces apoptosis.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Human CD27 protein was used as the immunogen for the recombinant CD27 antibody.
Limitation:
This recombinant CD27 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P26842