NSJ Bioreagents

CD68 Antibody

Product Code:
 
NSJ-V7007
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
LAM34-1
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the CD68 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC testing of FFPE human tonsil and CD68 antibody (clone LAM34-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
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IHC testing of FFPE human tonsil and CD68 antibody (clone LAM34-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

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CodeSizePrice
NSJ-V7007-20UG20 ug£264.00
Quantity:
NSJ-V7007-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
Application Note:
Titering of the CD68 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
CD68 plays a possible role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Purified CD68 protein was used as the immunogen for this CD68 antibody.
Limitation:
This CD68 antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P34810