NSJ Bioreagents

Ku70 + Ku80 Antibody

Product Code:
 
NSJ-V2128
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
KU729
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
Storage:
 
Store the Ku70 + Ku80 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
FACS testing of permeabilized human 293T cells with Ku70 + Ku80 antibody. Black=cells alone; Gray=isotype control; Green= AF488 conjugated Ku70 + Ku80 antibody.
2 / 3
FACS testing of permeabilized human K562 cells with Ku70 + Ku80 antibody. Black=cells alone; Green=isotype control; Red= PE conjugated Ku70 + Ku80 antibody.
3 / 3
Immunofluorescent staining of paraformaldehyde fixed human HeLa cells with Ku70 + Ku80 antibody (clone KU729, green) and Phalloidin cell membrane stain (red).
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FACS testing of permeabilized human 293T cells with Ku70 + Ku80 antibody. Black=cells alone; Gray=isotype control; Green= AF488 conjugated Ku70 + Ku80 antibody.
FACS testing of permeabilized human K562 cells with Ku70 + Ku80 antibody. Black=cells alone; Green=isotype control; Red= PE conjugated Ku70 + Ku80 antibody.
Immunofluorescent staining of paraformaldehyde fixed human HeLa cells with Ku70 + Ku80 antibody (clone KU729, green) and Phalloidin cell membrane stain (red).

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CodeSizePrice
NSJ-V2128-20UG20 ug£264.00
Quantity:
NSJ-V2128-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence/Immunocytochemistry: 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance.
Description:
This antibody recognizes a dimer of two proteins of 70kDa (Ku70) and ~80kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
2547
Immunogen:
Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.
Limitation:
This Ku70 + Ku80 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G purified monoclonal antibody
Species Reactivity :
Human