NSJ Bioreagents

p27 Antibody / p27Kip1

Product Code:
 
NSJ-V2436
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
KIP1/769
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the p27Kip1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 5
IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with p27Kip1 antibody (clone KIP1/769).
2 / 5
IHC: Formalin-fixed, paraffin-embedded human prostate cancer stained with p27Kip1 antibody (clone KIP1/769).
3 / 5
IHC: Formalin-fixed, paraffin-embedded rat colon stained with p27Kip1 antibody (clone KIP1/769).
4 / 5
Immunofluorescent staining of PFA-fixed human MCF7 cells with p27Kip1 antibody (clone KIP1/769, green) and Phalloidin (red).
5 / 5
SDS-PAGE analysis of purified, BSA-free p27Kip1 antibody (clone KIP1/769) as confirmation of integrity and purity.

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with p27Kip1 antibody (clone KIP1/769).
IHC: Formalin-fixed, paraffin-embedded human prostate cancer stained with p27Kip1 antibody (clone KIP1/769).
IHC: Formalin-fixed, paraffin-embedded rat colon stained with p27Kip1 antibody (clone KIP1/769).
Immunofluorescent staining of PFA-fixed human MCF7 cells with p27Kip1 antibody (clone KIP1/769, green) and Phalloidin (red).
SDS-PAGE analysis of purified, BSA-free p27Kip1 antibody (clone KIP1/769) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V2436-20UG20 ug£264.00
Quantity:
NSJ-V2436-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Application Note:
Optimal dilution of the p27Kip1 antibody to be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This mAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant human protein was used as the immunogen for the p27Kip1 antibody.
Limitation:
This p27Kip1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P46527