NSJ Bioreagents

EpCAM Antibody / Extracellular domain

Product Code:
 
NSJ-V3701
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
EPM17-5R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Western blot testing of human lung lysate with EpCAM antibody (clone EPM17-5R). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).~
2 / 2
IHC testing of FFPE human basal cell carcinoma and recombinant EpCAM antibody (cl
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Western blot testing of human lung lysate with EpCAM antibody (clone EPM17-5R). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).~
IHC testing of FFPE human basal cell carcinoma and recombinant EpCAM antibody (cl

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CodeSizePrice
NSJ-V3701-20UG20 ug£264.00
Quantity:
NSJ-V3701-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Titering of the recombinant EpCAM antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
EpCAM is frequently upregulated in carcinomas but is not expressed in cancers of non-epithelial origin. EpCAM expression is not limited to human colon carcinomas; in fact, EpCAM is expressed in a variety of human epithelial tissues, carcinomas, and progenitor and stem cells. [Wiki]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A portion of amino acids 100-224 (extracellular domain) was used as the immunogen for the recombinant EpCAM antibody.
Limitation:
This recombinant EpCAM antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P16422