NSJ Bioreagents

EpCAM Antibody

Product Code:
 
NSJ-V3117S
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
Epcam/1158
Regulatory Status:
 
RUO
Target Species:
  • Mouse
  • Rat
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the rat EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC: Formalin-fixed, paraffin-embedded rat colon stained with EpCAM antibody (Epcam/1158).~
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IHC: Formalin-fixed, paraffin-embedded rat lung stained with EpCAM antibody (Epcam/1158).
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IHC: Formalin-fixed, paraffin-embedded rat colon stained with EpCAM antibody (Epcam/1158).~
IHC: Formalin-fixed, paraffin-embedded rat lung stained with EpCAM antibody (Epcam/1158).

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CodeSizePrice
NSJ-V3117S-0.1ML0.1 ml£264.00
Quantity:
NSJ-V3117S-0.5ML0.5 ml£534.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1:50-1:100 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (3)
Application Note:
Optimal dilution of the rat EpCAM antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The epitopes of mAbs Epcam/1158 and Epcam/1159 monoclonal antibodies are different.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.
Format :
Bioreactor concentrate
Formulation :
Bioreactor concentrate with 0.05% sodium azide
Immunogen:
Recombinant full-length rat protein was used as the immunogen for the rat EpCAM antibody.
Limitation:
This rat EpCAM antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Unpurified high titer supernatant
Species Reactivity :
Rat and Mouse. Does not react with Human. Others species not known.
Uniprot #:
O55159