NSJ Bioreagents

CELA3B Antibody / Elastase 3B

Product Code:
 
NSJ-V7213
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
ELTS3B-2
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the Elastase 3B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC testing of FFPE rat pancreas with Elastase 3B antibody (clone ELTS3B-2). FFPE staining requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.~
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IHC testing of FFPE mouse
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IHC testing of FFPE rat pancreas with Elastase 3B antibody (clone ELTS3B-2). FFPE staining requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.~
IHC testing of FFPE mouse

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CodeSizePrice
NSJ-V7213-20UG20 ug£264.00
Quantity:
NSJ-V7213-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the Elastase 3B antibody should be determined by the researcher.
Description:
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.
Limitation:
This Elastase 3B antibody is available for research use only.
Purity:
Protein G
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P08861