NSJ Bioreagents

EpCAM Antibody

Product Code:
 
NSJ-V7264
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
EGP40/1555R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
IHC testing of FFPE human prostate carcinoma and recombinant EpCAM antibody (clone EGP40/1555R). HIER: steam sections in pH9 EDTA for 10-20 min.
2 / 4
IHC testing of FFPE human colon carcinoma and recombinant EpCAM antibody (clone EGP40/1555R). HIER: steam sections in pH9 EDTA for 10-20 min.
3 / 4
Flow cytometry testing of human MCF7 cells with recombinant EpCAM antibody (clone EGP40/1555R); Red=isotype control, Blue= recombinant EpCAM antibody.
4 / 4
SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone EGP40/1555R) as confirmation of integrity and purity.
❮❯

IHC testing of FFPE human prostate carcinoma and recombinant EpCAM antibody (clone EGP40/1555R). HIER: steam sections in pH9 EDTA for 10-20 min.
IHC testing of FFPE human colon carcinoma and recombinant EpCAM antibody (clone EGP40/1555R). HIER: steam sections in pH9 EDTA for 10-20 min.
Flow cytometry testing of human MCF7 cells with recombinant EpCAM antibody (clone EGP40/1555R); Red=isotype control, Blue= recombinant EpCAM antibody.
SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone EGP40/1555R) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V7264-20UG20 ug£264.00
Quantity:
NSJ-V7264-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Titering of the recombinant EpCAM antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A portion of amino acids 20-60 was used as the immunogen for the recombinant EpCAM antibody.
Limitation:
This recombinant EpCAM antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
P16422