NSJ Bioreagents

KRT19 Antibody / Cytokeratin 19

Product Code:
 
NSJ-V7144
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CTKN19-1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Keratin 19 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
Immunofluorescent staining of permeabilized human MCF7 cells with Cytokeratin 19 antibody (clone CTKN19-1, green) and Reddot nuclear stain (red).
2 / 3
Flow cytometry testing of methanol-fixed human MCF7 cells with Cytokeratin 19 antibody (clone CTKN19-1); Red=isotype control, Blue= Cytokeratin 19 antibody.
3 / 3
IHC testing of FFPE human ovarian carcinoma and Keratin 19 antibody (clone CTKN19-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
❮❯

Immunofluorescent staining of permeabilized human MCF7 cells with Cytokeratin 19 antibody (clone CTKN19-1, green) and Reddot nuclear stain (red).
Flow cytometry testing of methanol-fixed human MCF7 cells with Cytokeratin 19 antibody (clone CTKN19-1); Red=isotype control, Blue= Cytokeratin 19 antibody.
IHC testing of FFPE human ovarian carcinoma and Keratin 19 antibody (clone CTKN19-1). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

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CodeSizePrice
NSJ-V7144-20UG20 ug£264.00
Quantity:
NSJ-V7144-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
Titering of the Keratin 19 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Cytokeratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Cytokeratin 19 is a type I keratin. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically found in the periderm, the transiently superficial layer that envelops the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [Wiki]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Recombinant human KRT19 protein was used as the immunogen for the Keratin 19 antibody.
Limitation:
This Keratin 19 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P08727