CD43 Antibody

Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1) (2)

Optimal dilution of the CD43 antibody should be determined by the researcher.

1. For staining of formalin-fixed tissues, digest sections with Trypsin at 1mg/ml PBS, 15 min at room temp
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity). [UniProt]

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Immature pluripotent human leukemia K562 cells were used as the immunogen for the CD43 antibody.

This CD43 antibody is available for research use only.

Cell surface

PEG precipitation

Human

P16150