NSJ Bioreagents

Myeloid Cell Marker Antibody

Product Code:
 
NSJ-V2356
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
BM-1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the Myeloid cell marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Formalin/paraffin human tonsil stained with Myeloid cell marker antibody (BM-1).~
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SDS-PAGE Analysis of Purified, BSA-Free Myeloid Cell Marker Antibody (clone BM-1). Confirmation of Integrity and Purity of the Antibody.

Formalin/paraffin human tonsil stained with Myeloid cell marker antibody (BM-1).~
SDS-PAGE Analysis of Purified, BSA-Free Myeloid Cell Marker Antibody (clone BM-1). Confirmation of Integrity and Purity of the Antibody.

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CodeSizePrice
NSJ-V2356-20UG20 ug£264.00
Quantity:
NSJ-V2356-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Myeloid cell marker antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for staining of formalin/paraffin tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody recognizes a 183kDa protein with DNA-binding characteristics, which is identified as a myeloid cell specific antigen. Clone BM-1 antibody reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. This type of marker is useful in the identification of different levels of cellular differentiation. BM-1 and BM-2 antibodies react with early precursor and mature forms of human myeloid cells. This mAb is useful in the identification of myelogenous leukemias, distinguishing granulocytic sarcomas from lymphoid malignancies and also in the study of differentiation and transformation of human myeloid cells. The biological function of the marker protein is not clear, although it has been proposed that it may play a role in cell differentiation.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
Unknown
Immunogen:
Human peripheral blood mononuclear cells were used as the immunogen for this Myeloid cell marker antibody.
Limitation:
This Myeloid cell marker antibody is available for research use only.
Localization:
Cytoplasmic and nuclear
Purity:
Protein G affinity chromatography
Species Reactivity :
Human