NSJ Bioreagents

CD44 Antibody / HCAM

Product Code:
 
NSJ-RQ6270
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
7H7
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the HCAM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human A431 cells with HCAM antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) human A549 and 3) rat C6 cell lysate with HCAM antibody. Predicted molecular weight ~81 kDa.
3 / 3
Flow cytometry testing of human U-87 MG cells with HCAM antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HCAM antibody.
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Immunofluorescent staining of FFPE human A431 cells with HCAM antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human A549 and 3) rat C6 cell lysate with HCAM antibody. Predicted molecular weight ~81 kDa.
Flow cytometry testing of human U-87 MG cells with HCAM antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HCAM antibody.

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NSJ-RQ6270-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the HCAM antibody should be determined by the researcher.
Description:
CD44 is also known as HCAM, LHR and MC56. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids RFAGVFHVEKNGRYSISRTEAADLCKAF from the human protein were used as the immunogen for the HCAM antibody.
Limitation:
This HCAM antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
P16070