NSJ Bioreagents

RBPJ Antibody / RBP-JK

Product Code:
 
NSJ-RQ6277
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
6G4
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RBP-JK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 3
Flow cytometry testing of human U937 cells with RBP-JK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RBP-JK antibody.
2 / 3
Flow cytometry testing of mouse HEPA1-6 cells with RBP-JK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RBP-JK antibody.
3 / 3
Western blot testing of human 1) HEK293, 2) HepG2, 3) HeLa, 4) MCF7, 5) U-87 MG, 6) HL60, 7) rat NRK and 8) mouse smooth muscle tissue lysate with RBP-JK antibody. Predicted molecular weight ~56 kDa.
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Flow cytometry testing of human U937 cells with RBP-JK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RBP-JK antibody.
Flow cytometry testing of mouse HEPA1-6 cells with RBP-JK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RBP-JK antibody.
Western blot testing of human 1) HEK293, 2) HepG2, 3) HeLa, 4) MCF7, 5) U-87 MG, 6) HL60, 7) rat NRK and 8) mouse smooth muscle tissue lysate with RBP-JK antibody. Predicted molecular weight ~56 kDa.

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CodeSizePrice
NSJ-RQ6277-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the RBP-JK antibody should be determined by the researcher.
Description:
Recombination signal binding protein for immunoglobulin kappa J region is a protein that in humans is encoded by the RBPJ gene. It is mapped to 4p15.2. The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids K41-Q467) was used as the immunogen for the RBP-JK antibody.
Limitation:
This RBP-JK antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q06330