NSJ Bioreagents

MMUT Antibody / Methylmalonyl Coenzyme A mutase

Product Code:
 
NSJ-RQ5944
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
2D6
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the MMUT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 5
IHC staining of FFPE human rectal cancer with MMUT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human rectal cancer with MMUT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human breast cancer with MMUT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
IHC staining of FFPE rat intestine with MMUT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 5
Western blot testing of human 1) HeLa, 2) K562, 3) HEK293, 4) PC-3, 5) Caco-2, 6) Raji, 7) rat PC-12 and 8) mouse NIH 3T3 lysate with MMUT antibody. Predicted molecular weight ~83 kDa.
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IHC staining of FFPE human rectal cancer with MMUT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with MMUT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with MMUT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with MMUT antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) K562, 3) HEK293, 4) PC-3, 5) Caco-2, 6) Raji, 7) rat PC-12 and 8) mouse NIH 3T3 lysate with MMUT antibody. Predicted molecular weight ~83 kDa.

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NSJ-RQ5944-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml
Application Note:
Optimal dilution of the MMUT antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Methylmalonyl-CoA mutase (MUT) is a mitochondrial enzyme that catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA. This gene is mapped to 6p12.3. MUT requires a vitamin B12-derived prosthetic group, adenosylcobalamin (commonly referred to as AdoCbl), to function. And the product of this enzyme, succinyl-CoA, is a key molecule of the TCA cycle.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids LHQQQPLHPEWAALAKKQLKGKNPEDLIWHTPE from the human protein were used as the immunogen for the MMUT antibody.
Limitation:
This MMUT antibody is available for research use only.
Localization:
Cytoplasmic (mitochondria)
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P22033