NSJ Bioreagents

CD31 Antibody / PECAM-1

Product Code:
 
NSJ-RQ5938
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
2D4
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PECAM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 4
IHC staining of FFPE human breast cancer with PECAM1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human lung cancer with PECAM1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
IHC staining of frozen human placenta with PECAM1 antibody.
4 / 4
Western blot testing of human 1) Jurkat and 2) placenta lysate with PECAM1 antibody. Expected molecular weight: 83-130 kDa depending on level of glycosylation.
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IHC staining of FFPE human breast cancer with PECAM1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with PECAM1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of frozen human placenta with PECAM1 antibody.
Western blot testing of human 1) Jurkat and 2) placenta lysate with PECAM1 antibody. Expected molecular weight: 83-130 kDa depending on level of glycosylation.

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NSJ-RQ5938-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE & Frozen): 1-2ug/ml
Application Note:
Optimal dilution of the PECAM1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
CD31 also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a protein that in human is encoded by the PECAM1 gene. Encoded protein is a member of the immunoglobulin superfamily, CD31 is mapped to 17q23.3. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is demonstrated that CD31 expression on human PBSCs may positively affect both neutrophil and platelet engraftment. Meanwhile, CD31 is involved in leukocyte migration and angiogenesis, which are key components of venous thrombus resolution.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Q28-G382) was used as the immunogen for the PECAM1 antibody.
Limitation:
This PECAM1 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P16284