Further Information
Proofreading exoribonuclease, Guanine-N7 methyltransferase, Non-structural protein 14, NSP14.
IHC: 0.5 μg/mL .
Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. All other applications and species not yet tested
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019?20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP14 (ExoN) is an enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity (3,4,5). It acts as a proofreading exoribonuclease for RNA replication, thereby lowering The sensitivity of the virus to RNA mutagens(6,7,8).
- Gorbalenya. bioRxiv: 2020.
- Hui et al. Int J Infect Dis. 2020;91:264-266.
- Minskaia et. al. Proc. Natl. Acad. Sci. U.S.A 2006; 103:5108-5113
- Bouvet et. al. PLoS Pathog. 2010; 6:E1000863-E1000863
- Bouvet et. al. Proc. Natl. Acad. Sci. U.S.A. 2012;109:9372-9377
- Denison et. al. RNA Biol. 2011;8:270-279
- Smith et. al. PLoS Pathog. 2013;9:e1003565-e1003565
- Agostini et. al. MBio 2018; 9:0-0
SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted reactivity based on immunogen sequence: SARS-CoV NSP14 (ExoN) protein: (79%).
Anti-SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody (9185) was raised against a peptide corresponding to 14 amino acids near the center of SARS-CoV-2 (COVID-19) NSP14 (ExoN) protein.
The immunogen is located between 120-170 amino acids of SARS-CoV-2 (COVID-19) NSP14 (ExoN).
43740578
ORF1a polyprotein;ORF1ab polyprotein
ORF1ab
Severe acute respiratory syndrome coronavirus 2
Liquid
YP_009725309.1
1802476817
SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is affinity chromatography purified via peptide column.
Infectious Disease,COVID-19
P0DTC1
Optimal dilutions for each application to be determined by the researcher.