ProSci

HACE1 Antibody

Product Code:
 
PSI-23-886
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using HACE1 antibody (23-886) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded human liver cancer using HACE1 antibody (23-886) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human placenta using HACE1 antibody (23-886) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat testis using HACE1 antibody (23-886) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using HACE1 antibody (23-886) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using HACE1 antibody (23-886) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human liver cancer using HACE1 antibody (23-886) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using HACE1 antibody (23-886) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using HACE1 antibody (23-886) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HACE1 antibody (23-886) at dilution of 1:100 (40x lens).

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PSI-23-886-50uL50uL£433.00
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Further Information

Additional Names:
HACE1, KIAA1320
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a HECT domain and ankyrin repeat-containing ubiquitin ligase. The encoded protein is involved in specific tagging of target proteins, leading to their subcellular localization or proteasomal degradation. The protein is a potential tumor suppressor and is involved in the pathophysiology of several tumors, including Wilm's tumor.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HACE1 (NP_065822.2).
NCBI Gene ID #:
57531
NCBI Official Name:
HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1
NCBI Official Symbol:
HACE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 102kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle
Swissprot #:
Q8IYU2
User NOte:
Optimal dilutions for each application to be determined by the researcher.