ProSci

SUCLG2 Antibody

Product Code:
 
PSI-23-768
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using SUCLG2 antibody (23-768) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 3
Immunofluorescence analysis of C6 cells using SUCLG2 Polyclonal Antibody (23-768) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of U-2 OS cells using SUCLG2 Polyclonal Antibody (23-768) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using SUCLG2 antibody (23-768) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of C6 cells using SUCLG2 Polyclonal Antibody (23-768) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using SUCLG2 Polyclonal Antibody (23-768) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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CodeSizePrice
PSI-23-768-50uL50uL£433.00
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Further Information

Additional Names:
G-SCS, GBETA, GTPSCS, succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial, GTP-specific succinyl-CoA synthetase beta subunit, GTP-specific succinyl-CoA synthetase subunit beta, SCS-betaG, succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial, succinyl-CoA ligase, GDP-forming, beta chain, mitochondrial, succinyl-CoA synthetase, beta-G chain
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
This gene encodes a GTP-specific beta subunit of succinyl-CoA synthetase. Succinyl-CoA synthetase catalyzes the reversible reaction involving the formation of succinyl-CoA and succinate. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 5 and 12.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 50-300 of human SUCLG2 (NP_003839.2).
NCBI Gene ID #:
8801
NCBI Official Name:
succinate-CoA ligase GDP-forming beta subunit
NCBI Official Symbol:
SUCLG2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 47kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q96I99
User NOte:
Optimal dilutions for each application to be determined by the researcher.